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amaxa tm mef 1 nucleofector tm kit  (Lonza)


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    Lonza amaxa tm mef 1 nucleofector tm kit
    Amaxa Tm Mef 1 Nucleofector Tm Kit, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nucleofector+mef+1+kit/4d+nucleofector/pmc11094137-395-38-45
    Average 90 stars, based on 1 article reviews
    amaxa tm mef 1 nucleofector tm kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Transfection:

    Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition
    Article Snippet: To ectopically express miR-21 , the GFP sequence of plasmid PMIRH21PA-1-GVO-SBI (System Biosciences), which codes for the microRNA precursor of miR-21 , was replaced by the sequence of the gene encoding tdTomato fluorescent protein. .. 1.5x10ˆ6 primary neonatal human dermal fibroblast (NHDF) (ATCC, CRL-2522) were transfected using the Nucleofector MEF 1 Kit (VPD-1004, Lonza) with 6μg of plasmid MIP 247 CoMiP 4in1 and 3ug of pCXLE-hMLN (63726 and 27079, Addgene). .. Cells were pulsed with T-020 or N-024 program using Nucleofector 2b (AAB-1001, Lonza), and were plated on Matrigel-coated plates with fibroblast medium composed of DMEM high glucose (11965092, Thermo Fisher Scientific), 10% HyClone Fetal Bovine Serum (SV30160.03HI GE Healthcare), 0.1mM sodium butyrate (B5887-250MG, Sigma Aldrich) and 64 μg/mL ascorbic acid (A8960, Sigma Aldrich).

    Plasmid Preparation:

    Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition
    Article Snippet: To ectopically express miR-21 , the GFP sequence of plasmid PMIRH21PA-1-GVO-SBI (System Biosciences), which codes for the microRNA precursor of miR-21 , was replaced by the sequence of the gene encoding tdTomato fluorescent protein. .. 1.5x10ˆ6 primary neonatal human dermal fibroblast (NHDF) (ATCC, CRL-2522) were transfected using the Nucleofector MEF 1 Kit (VPD-1004, Lonza) with 6μg of plasmid MIP 247 CoMiP 4in1 and 3ug of pCXLE-hMLN (63726 and 27079, Addgene). .. Cells were pulsed with T-020 or N-024 program using Nucleofector 2b (AAB-1001, Lonza), and were plated on Matrigel-coated plates with fibroblast medium composed of DMEM high glucose (11965092, Thermo Fisher Scientific), 10% HyClone Fetal Bovine Serum (SV30160.03HI GE Healthcare), 0.1mM sodium butyrate (B5887-250MG, Sigma Aldrich) and 64 μg/mL ascorbic acid (A8960, Sigma Aldrich).



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    Image Search Results


    Journal: Molecular Cell

    Article Title: Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition

    doi: 10.1016/j.molcel.2019.03.041

    Figure Lengend Snippet:

    Article Snippet: 1.5x10ˆ6 primary neonatal human dermal fibroblast (NHDF) (ATCC, CRL-2522) were transfected using the Nucleofector MEF 1 Kit (VPD-1004, Lonza) with 6μg of plasmid MIP 247 CoMiP 4in1 and 3ug of pCXLE-hMLN (63726 and 27079, Addgene).

    Techniques: Recombinant, Produced, Knock-Out, TaqMan Assay, Labeling, Staining, Flow Cytometry, Western Blot, Clarification Assay, Sample Prep, Sequencing, Derivative Assay, Next-Generation Sequencing, Expressing, Fractionation, Activation Assay, Nucleic Acid Electrophoresis, CRISPR, Plasmid Preparation, Modification, shRNA, Software